Volume 7, Issue 2, April 2019, Page: 39-48
Atropisomeric Separation of PCB-95 by HPLC
Prabha Ranasinghe, Environmental Toxicology Program, Clemson University, Clemson, USA; Department of Environmental Engineering and Earth Sciences, Clemson University, Clemson, USA
Christopher Olivares, Department of Environmental Engineering and Earth Sciences, Clemson University, Clemson, USA
William Champion Jr, Chiral Technologies Inc., West Chester, USA
Cindy Lee, Environmental Toxicology Program, Clemson University, Clemson, USA; Department of Environmental Engineering and Earth Sciences, Clemson University, Clemson, USA
Received: Feb. 3, 2019;       Accepted: Mar. 20, 2019;       Published: Jun. 29, 2019
DOI: 10.11648/j.sjc.20190702.12      View  186      Downloads  64
Abstract
2,2’,3,5’,6-Pentachlorobiphenyl (PCB-95) is an environmentally significant chiral PCB, of which enantioselective toxicity, biodegradation and chiral stability studies have been limited to date, as no commercially available enantiomers exist for PCB-95 and due to the lack of an efficient preparatory chiral separation method. A selective, sensitive, and rapid high-performance liquid chromatography with UV detection (HPLC-UV) method has been developed and validated for the chromatographic separation and quantitation of PCB-95 enantiomers. In this study, we resolved enantiomers of PCB-95 using a cellulose tris (4-methylbenzoate) Chiralcel OJ- H column. After evaluating mobile phase compositions and temperatures, optimum separation and detection were obtained with isocratic 100% n-hexane as the mobile phase, a column temperature of 20°C, a flow rate of 1 mL/min, and a detection wavelength of 280 nm. The total run time was 8 minutes. Enantiomer purity was confirmed using enantioselective gas capillary chromatography-electron capture detection. The developed method was validated as per International Conference on Harmonization (ICH) guidelines with respect to limit of detection, limit of quantification, precision, linearity, robustness and ruggedness.
Keywords
Enantioselective Studies, 2, 2’, 3, 5’, 6-Pentachlorobiphenyl, Chiralcel OJ - H, Liquid Chromatography
To cite this article
Prabha Ranasinghe, Christopher Olivares, William Champion Jr, Cindy Lee, Atropisomeric Separation of PCB-95 by HPLC, Science Journal of Chemistry. Vol. 7, No. 2, 2019, pp. 39-48. doi: 10.11648/j.sjc.20190702.12
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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